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href="/article.html">技術(shù)文章</a> > 大鼠低氧誘導(dǎo)因子1αelisa試劑盒性能優(yōu)勢(shì)</span></div> <div id="vzbcvzp" class="about"> <div class="vzbcvzp" id="newscont"> <h1 class="newsname">大鼠低氧誘導(dǎo)因子1αelisa試劑盒性能優(yōu)勢(shì)</h1> <li id="vzbcvzp" class="time">更新時(shí)間：2023-02-09 瀏覽次數(shù)：1320 </li> <ul class="dcont"> <div>　　<a href="http://www.pixur.cn/Products-37030636.html" target="_blank">大鼠低氧誘導(dǎo)因子1αelisa試劑盒</a>標(biāo)準(zhǔn)品和樣品中的目標(biāo)蛋白與固定化的抗體結(jié)合，移除未結(jié)合的底物后，加入目標(biāo)蛋白特異性的生物素化的檢測(cè)抗體，經(jīng)過洗滌，向反應(yīng)孔中加入的鏈霉親和素-HRP偶聯(lián)物。洗滌移除所有未結(jié)合的鏈霉親和素-HRP試劑，加入HRP底物溶液，產(chǎn)生有色產(chǎn)物，顏色的深淺與目標(biāo)蛋白的的含量成正比。<br /> </div><div>　　在檢測(cè)大鼠低氧誘導(dǎo)因子1αelisa試劑盒時(shí)，需要you質(zhì)的試劑，良好狀態(tài)的儀器，排除各種因素的干擾，正確操作是保證檢測(cè)結(jié)果準(zhǔn)確可靠的必要條件。<br /> </div><div>　　1、加樣后及時(shí)放人孵箱，標(biāo)本較多時(shí)，要分批操作。按說明步驟嚴(yán)格控制操作時(shí)間，防止孵育時(shí)間人為延長(zhǎng)，導(dǎo)致非特異性結(jié)合緊附于反應(yīng)孔周圍，難以清洗。<br /> </div><div>　　2、顯色劑盡量在臨用前配制，堅(jiān)持不用過期顯色劑，肉眼可見淺藍(lán)色的TMB顯色劑不用。<br /> </div><div>　　3、加酶試劑后用吸水紙?jiān)诿笜?biāo)板表面輕拭吸干。<br /> </div><div>　　4、合理安排檢測(cè)量，以免反應(yīng)板過多造成洗板等待時(shí)間長(zhǎng)。<br /> </div><div>　　性能優(yōu)勢(shì)：<br /> </div><div>　　1、包被：采用國(guó)內(nèi)精度的包被機(jī)，板內(nèi)誤差和板間誤差在5%之內(nèi)；<br /> </div><div>　　2、樣本稀釋液：我公司提供的樣本稀釋液可有效消除血清中某些成份的影響，準(zhǔn)確度更高。<br /> </div><div>　　3、其特異性來自抗體或抗原的選擇性，抗原抗體的結(jié)合實(shí)質(zhì)上只發(fā)生在抗原的抗原決定簇與抗體的抗原結(jié)合位點(diǎn)之間，由于兩者在化學(xué)結(jié)構(gòu)和空間構(gòu)型上呈互補(bǔ)關(guān)系，所以抗原抗體反應(yīng)具有高度的特異性。</div> </ul> <div class="vzbcvzp" id="news_sx"> <li id="vzbcvzp" class="sx_left">上一篇：<a href="/Article-3289699.html">羊ELISA試劑盒是種常見的免疫學(xué)實(shí)驗(yàn)方法</a><br />下一篇：<a href="/Article-3238625.html">人ELISA試劑盒的樣本處理及要求</a></li> <li id="vzbcvzp" class="sx_right"><a href="/article.html">返回</a></li> </div> </div> </div> </div> </div> <div class="vzbcvzp" id="clear"></div> <div id="vzbcvzp" class="footer"> <div id="vzbcvzp" class="w1200"> <div id="vzbcvzp" class="footaddress"> <i class="flogo"></i> <div id="vzbcvzp" 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