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id="ny_biaoti"><span>您現(xiàn)在的位置：<a href="/">首頁</a> > <a href="/article.html">技術(shù)文章</a> > 人ELISA試劑盒的樣本處理及要求</span></div> <div id="vzbcvzp" class="about"> <div class="vzbcvzp" id="newscont"> <h1 class="newsname">人ELISA試劑盒的樣本處理及要求</h1> <li id="vzbcvzp" class="time">更新時間：2023-01-03 瀏覽次數(shù)：1408 </li> <ul class="dcont"> <div>　　<a href="http://www.pixur.cn/Products-36986414.html" target="_blank">人ELISA試劑盒</a>是一種夾心ELISA試劑盒，具有高度的靈敏度和特異性，用于檢測人血清、血漿、組織勻漿和其他生物液體中的PDPN。它主要是用于檢測血漿，血清及相關(guān)液體等樣本。例如灌洗液、腦脊液、血清、血漿、尿液、胸腹水、細胞培養(yǎng)上清、組織勻漿等標本。<br /> </div><div>　　人ELISA試劑盒樣本處理及要求：<br /> </div><div>　　1、血清：室溫血液自然凝固10-20分鐘，離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清，保存過程中如出現(xiàn)沉淀，應(yīng)再次離心。<br /> </div><div>　　2、血漿：應(yīng)根據(jù)標本的要求選擇EDTA或檸檬酸鈉作為抗凝劑，混合10-20分鐘后，離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清，保存過程中如有沉淀形成，應(yīng)該再次離心。<br /> </div><div>　　3、尿液：用無菌管收集，離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清，保存過程中如有沉淀形成，應(yīng)再次離心。胸腹水、腦脊液參照實行。<br /> </div><div>　　4、細胞培養(yǎng)上清：檢測分泌性的成份時，用無菌管收集。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。檢測細胞內(nèi)的成份時，用PBS(PH7.2-7.4)稀釋細胞懸液，細胞濃度達到100萬/ml左右。通過反復(fù)凍融，以使細胞破壞并放出細胞內(nèi)成份。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。保存過程中如有沉淀形成，應(yīng)再次離心。<br /> </div><div>　　5、組織標本：切割標本后，稱取重量。加入一定量的PBS，PH7.4。用液氮迅速冷凍保存?zhèn)溆?。標本融化后仍然保?-8℃的溫度。加入一定量的PBS(PH7.4)，用手工或勻漿器將標本勻漿充分。離心20分鐘左右(2000-3000轉(zhuǎn)/分)。仔細收集上清。分裝后一份待檢測，其余冷凍備用。<br /> </div><div>　　6、標本采集后盡早進行提取，提取按相關(guān)文獻進行，提取后應(yīng)盡快進行實驗。若不能馬上進行試驗，可將標本放于-20℃保存，但應(yīng)避免反復(fù)凍融.<br /> </div><div>　　7、不能檢測含NaN3的樣品，因NaN3抑制辣根過氧化物酶的(HRP)活性。</div> </ul> <div class="vzbcvzp" id="news_sx"> <li id="vzbcvzp" class="sx_left">上一篇：<a href="/Article-3261244.html">大鼠低氧誘導(dǎo)因子1αelisa試劑盒性能優(yōu)勢</a><br />下一篇：<a href="/Article-3219660.html">人γ肽PγELISA試劑盒的操作步驟</a></li> <li id="vzbcvzp" class="sx_right"><a href="/article.html">返回</a></li> </div> </div> </div> </div> </div> <div class="vzbcvzp" id="clear"></div> <div id="vzbcvzp" class="footer"> <div id="vzbcvzp" class="w1200"> 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