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id=tagContent0><p><strong><strong>小鼠總膽汁酸 TBA ELISA試劑盒</strong><br/>實(shí)驗(yàn)材料與試劑配制:</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong><span style="font-weight:700;">試劑</span></strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>(1) 包被緩沖液(PH9.60.05M碳酸鹽緩沖液):</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>Na2CO3 1.59克</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>NaHCO3 2.93克</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>加蒸餾水至1000ml</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>(2) 洗滌緩沖液(PH7.4PBS):0.15M</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>KH2PO4 0.2克</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>Na2HPO4·12H2O 2.9克</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>NaCl 8.0克</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>KCl 0.2克</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>Tween-20 0.05% 0.5ml</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>加蒸餾水至1000ml</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>(3) 稀釋液:</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>牛血清白蛋白(BSA) 0.1克</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>加洗滌緩沖液至100ml</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>或以羊血清、兔血清等血清與洗滌液配成5~10%使用。</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>(4) 終止液(3M H2SO4):</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>蒸餾水178.3ml，逐滴加入濃硫酸(98%)21.7ml。</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>(5) 底物緩沖液(PH5.0磷酸棗檸檬酸):</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>0.2MNa2HPO4(28.4克/L)25.7ml</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>0.1M檸檬酸(19.2克/L) 24.3ml</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>加蒸餾水50ml。</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>(6) TMB(四甲基聯(lián)苯胺)使用液:</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>TMB(10mg/5ml無水乙醇)0.5ml</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>底物緩沖液(PH5.5) 10ml</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>0.75%H2O2 32μl</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>(7) ABTS使用液:</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>ABTS 0.5mg</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>底物緩沖液(PH5.5) 1ml</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>3%H2O2 2μl</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, sans-serif;font-size:14px;white-space:normal;background-color:#FFFFFF;padding:0px;line-height:24px;text-indent:2em;zoom:1;"><strong>(8) 抗原、抗體和酶標(biāo)記抗體。</strong></p><p style="margin-top:0px;margin-bottom:15px;color:#333333;font-family:arial, 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